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dc.coverage.spatialNEW YORK
dc.creatorHuebner, Holger
dc.creatorYoung-Anze, Manuel
dc.creatorBuchholz, Rainer
dc.creatorAcevedo-Gutiérrez, Cristian
dc.creatorWeinstein-Oppenheimer, Caroline
dc.creatorBrown-González, Donald
dc.date.accessioned2017-04-27T18:52:48Z
dc.date.available2017-04-27T18:52:48Z
dc.date.issued2009
dc.identifier.issn1615-7591
dc.identifier.urihttp://hdl.handle.net/10533/197755
dc.description.abstractThe use of fibrin in tissue engineering has greatly increased over the last 10 years. The aim of this research was to develop a mathematical model to relate the microcapsule-size and cell-load to growth and oxygen depletion. Keratinocytes were isolated from rat skins and microencapsulated dropping fibrinogen and thrombin solutions. The cell growth was measured with MTT-assay and confirmed using histochemical technique. The oxygen was evaluated using a Clark sensor. It was found that Fick-Monod model explained the cell growth for the first 48 h, but overestimated the same thereafter. It was necessary to add a logistic equation to reach valid results. In relation to the preferred implant alternative, when considering large initial cell loads, the possibility to implant small loads of fast-growing cells arises from the simulations. In relation to the microcapsule size, it was found that a critical diameter could be established from which cell growth velocity is about the same.
dc.language.isoENG
dc.publisherSPRINGER
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.titleA mathematical model for the design of fibrin microcapsules with skin cells
dc.typeArticulo
dc.bibliographicCitation.stpage341
dc.bibliographicCitation.endpage351
dc.identifier.folioD02I1009
dc.country.isoDEU
dc.description.citasisi13
dc.description.conicytprogramFONDEF
dc.description.emailcristian.acevedo@usm.cl
dc.description.investmentarticleCONICYT from Chile by: FONDEF [DO211009]; Doctoral Scholarship for Cristian Acevedo [D-21050588]
dc.description.number3
dc.description.volume32
dc.identifier.isiWOS:000264464000006
dc.relation.projectidinfo:eu-repo/grantAgreement/Fondef/D02I1009
dc.relation.setinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.title.journalBIOPROCESS AND BIOSYSTEMS ENGINEERING (PRINT)
dc.title.journalabbreviationBioprocess. Biosyst. Eng.
dc.type.driverinfo:eu-repo/semantics/article
dc.description.shortconicytprogramFONDEF
dc.identifier.eissn0
dc.description.agradThe authors thank CONICYT from Chile by: FONDEF Grant (# DO211009) and Doctoral Scholarship for Cristian Acevedo (# D-21050588).
dc.type.openaireinfo:eu-repo/semantics/publishedVersion


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