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dc.coverage.spatialPuerto Varases_CL
dc.creatorFuentealba-Alday, Rodrigo Esteban
dc.creatorVasquez, Manuel
dc.creatorFernandez, Sebastián
dc.creatorVega-Letter, Ana María
dc.creatorKurte, Mónica
dc.creatorMoya, Ivón
dc.creatorGauthier-Abeliuk, Melanie
dc.creatorCarrion, Flavio
dc.creatorFigueroa, Fernando
dc.description.abstractMesenchymal Stem Cells (MSC) are multipotent cells that show strong immunosuppressive properties after inflammatory environment sensing. This sensing capacity could be related to the action of neurotransmitters. γ-aminobutyric acid (GABA) is a potent anti-inflammatory molecule that inhibits: (1) T-cell proliferation in vitro, (2) the secretion of pro-inflammatory cytokines by activated macrophages, and (3) antigen presenting cells function. GABA is synthesized from glutamic acid by glutamic acid decarboxylase (GAD). We have previously shown that pro-inflammatory cytokines increase GAD expression and enhance MSC-mediated immunosuppression in vitro. Moreover, expression of GAD increases immunosuppressive properties of murine MSC in the absence of proinflammatory stimulus (CD4+ T-cell co-culture). Here, we evaluated the effect of GAD expression on splenic T-cell proliferation, and analyzed levels of the soluble immunosuppressors GABA and nitric oxide, both in pure MSC cultures and in MSC/splenocytes co-cultures. Expression of GAD also increased the immunosuppressive properties of MSC to splenocytes stimulated with Anti-CD3/Anti-CD28. Levels of GABA, but not nitric oxide, were increased in pure MSC transfected with GAD. Surprisingly, increased nitric oxide levels were detected in co-culture supernatants of GAD-expressing MSC, and these levels were comparable to those obtained using licensed MSC. These results may indicate interplay between GABAergic and nitric oxide signaling in the setting of MSC-mediated immunosuppressiones_CL
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
dc.titleGABAergic activation of Mesenchymal Stem Cells increases their immunosuppressive properties via increased nitric oxide and GABAes_CL
dc.relation.namecongReunión anual de la Sociedad de Biología de Chilees_CL

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