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dc.coverage.spatialPuerto Varases_CL
dc.creatorFuentealba-Alday, Rodrigo Esteban
dc.creatorVasquez, Manuel
dc.creatorFernandez, Sebastián
dc.creatorVega-Letter, Ana María
dc.creatorKurte, Mónica
dc.creatorMoya, Ivón
dc.creatorGauthier-Abeliuk, Melanie
dc.creatorCarrion, Flavio
dc.creatorFigueroa, Fernando
dc.date.accessioned2018-07-18T13:41:14Z
dc.date.available2018-07-18T13:41:14Z
dc.date.issued2015es_CL
dc.identifier.urihttp://hdl.handle.net/10533/218747
dc.description.abstractMesenchymal Stem Cells (MSC) are multipotent cells that show strong immunosuppressive properties after inflammatory environment sensing. This sensing capacity could be related to the action of neurotransmitters. γ-aminobutyric acid (GABA) is a potent anti-inflammatory molecule that inhibits: (1) T-cell proliferation in vitro, (2) the secretion of pro-inflammatory cytokines by activated macrophages, and (3) antigen presenting cells function. GABA is synthesized from glutamic acid by glutamic acid decarboxylase (GAD). We have previously shown that pro-inflammatory cytokines increase GAD expression and enhance MSC-mediated immunosuppression in vitro. Moreover, expression of GAD increases immunosuppressive properties of murine MSC in the absence of proinflammatory stimulus (CD4+ T-cell co-culture). Here, we evaluated the effect of GAD expression on splenic T-cell proliferation, and analyzed levels of the soluble immunosuppressors GABA and nitric oxide, both in pure MSC cultures and in MSC/splenocytes co-cultures. Expression of GAD also increased the immunosuppressive properties of MSC to splenocytes stimulated with Anti-CD3/Anti-CD28. Levels of GABA, but not nitric oxide, were increased in pure MSC transfected with GAD. Surprisingly, increased nitric oxide levels were detected in co-culture supernatants of GAD-expressing MSC, and these levels were comparable to those obtained using licensed MSC. These results may indicate interplay between GABAergic and nitric oxide signaling in the setting of MSC-mediated immunosuppressiones_CL
dc.language.isoenges_CL
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.rightsinfo:eu-repo/semantics/openAccesses_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.titleGABAergic activation of Mesenchymal Stem Cells increases their immunosuppressive properties via increased nitric oxide and GABAes_CL
dc.typePonencia
dc.identifier.folio1130482es_CL
dc.country.isoChilees_CL
dc.relation.ncongress58es_CL
dc.relation.projectidinfo:eu-repo/grantAgreement//1130482es_CL
dc.relation.setinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93486
dc.type.driverinfo:eu-repo/semantics/lecture
dc.relation.namecongReunión anual de la Sociedad de Biología de Chilees_CL
dc.date.startcongress2015-11-23
dc.date.endcongress2015-11-25


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