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dc.creatorReyes, D
dc.creatorSalazar-Onfray, Flavio
dc.creatorEspinoza, E
dc.creatorPereda, Cristián
dc.creatorCastellon, E
dc.creatorValdevenito, R
dc.creatorHuidobro, C
dc.creatorBecker, María Ines
dc.creatorLladser, A
dc.creatorLópez, Mercedes N
dc.date.accessioned2018-08-13T18:06:47Z
dc.date.available2018-08-13T18:06:47Z
dc.date.issued2013es_CL
dc.identifier.urihttp://hdl.handle.net/10533/219346
dc.description.abstractBackground: Recently, we produced a tumour antigen-presenting cells (TAPCells) vaccine using a melanoma cell lysate, called TRIMEL, as an antigen source and an activation factor. Tumour antigen-presenting cells induced immunological responses and increased melanoma patient survival. Herein, we investigated the effect of TAPCells loaded with prostate cancer cell lysates (PCCL) as an antigen source, and TRIMEL as a dendritic cell (DC) activation factor; which were co-injected with the Concholepas concholepas haemocyanin (CCH) as an adjuvant on castration-resistant prostate cancer (CRPC) patients. Methods: The lysate mix capacity, for inducing T-cell activation, was analysed by flow cytometry and Elispot. Delayed-type hypersensitivity (DTH) reaction against PCCL, frequency of CD8(+) memory T cells (Tm) in blood and prostate-specific antigen (PSA) levels in serum were measured in treated patients. Results: The lysate mix induced functional mature DCs that were capable of activating PCCL-specific T cells. No relevant adverse reactions were observed. Six out of 14 patients showed a significant decrease in levels of PSA. DTH+ patients showed a prolonged PSA doubling-time after treatment. Expansion of functional central and effector CD8(+) Tm were detected. Conclusion: Treatment of CRPC patients with lysate-loaded TAPCells and CCH as an adjuvant is safe: generating biochemical and memory immune responses. However, the limited number of cases requires confirmation in a phase II clinical trial. Keywords Author Keywords:dendritic cells; prostate cancer vaccine; Concholepas haemocyanin; PSA; memory T cells; DTHes_CL
dc.language.isoeng
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/23989944es_CL
dc.rightsinfo:eu-repo/semantics/openAccesses_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.titleTumour cell lysate-loaded dendritic cell vaccine induces biochemical and memory immune response in castration-resistant prostate cancer patientses_CL
dc.typeArticulo
dc.bibliographicCitation.endpage
dc.identifier.folio1130324es_CL
dc.relation.projectidinfo:eu-repo/grantAgreement//1130324es_CL
dc.relation.setinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93477
dc.rights.driverinfo:eu-repo/semantics/openAccess
dc.title.journalBritish Journal Of Canceres_CL
dc.type.driverinfo:eu-repo/semantics/article
dc.type.openaireinfo:eu-repo/semantics/publishedVersion


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