Wnt signaling in the processes of segmentation and elongation in the beetle Tribolium castaneum
Author
Macaya-Ramos, ConstanzaNuñez, Viviana A
Sarrazin-Castillo, Andrés Fernando
Abstract
Introduction: In most arthropods segments form sequentially from a posterior growth zone in
an anterior to posterior fashion, just like vertebrate somites arise from presomitic mesoderm. In
both phyla, segmentation relies on the dynamic expression of cyclic genes belonging to the
same signaling pathways. However, much of what we know about segmentation in arthropods
comes from RNAi-based loss of function analysis, with the corresponding unwan...
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Introduction: In most arthropods segments form sequentially from a posterior growth zone in
an anterior to posterior fashion, just like vertebrate somites arise from presomitic mesoderm. In
both phyla, segmentation relies on the dynamic expression of cyclic genes belonging to the
same signaling pathways. However, much of what we know about segmentation in arthropods
comes from RNAi-based loss of function analysis, with the corresponding unwanted early
effects. Furthermore, it has not been always possible to relate the phenotype obtained with the
specific affected part of the process.
Material and Methods: Transgenic nuclear GFP-line, embryo culture (dissected and bisected
Tribolium eggs) and in-situ hybridization, to test the inhibition (IWP-3) and activation (LiCl) of
Wnt signaling pathway during specific time intervals.
Results: The activation and inhibition of Wnt signaling pathway affect axis elongation in
dissected and bisected embryos, as well as partially disrupts convergent movements toward the
ventral midline. At the same time we are analyzing the expression patterns of the two oscillatory
genes known in Tribolium (odd-skipped and even-skipped), by in-situ hybridization.
Discussion: By incubating the embryos with different drugs at specific time intervals during
segmentation, we want to determine the role of different signaling pathways, Wnt pathway
included, during embryonic elongation and segmentation, compared to what happens in
vertebrates and other arthropods.
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Date de publicación
2014Associated congress
28
Metadata
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