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dc.coverage.spatialPucónes_CL
dc.creatorFarias, A
dc.creatorCéspedes, C
dc.creatorMoraga, F
dc.creatorJara, C
dc.creatorQuíroz-Cortéz, Andrés-Eduardo
dc.creatorHormazabal, Emilio
dc.date.accessioned2019-05-28T19:35:19Z
dc.date.available2019-05-28T19:35:19Z
dc.date.issued2017es_CL
dc.identifier.urihttp://hdl.handle.net/10533/235724
dc.description.abstractThe Amaryllidaceae family has been largely mentioned in the use of traditional medicine. A particular characteristic of this family is the presence of a large group of isoquinolinic alkaloids, most of them absent in any other plant family. Currently, more than 500 alkaloids have been isolated from plants from Amaryllidaceae, and although their structures vary considerably, these alkaloids are related biogenetically. The resistance of microorganisms against antibiotic is a permanent incentive to search for antimicrobial agents from natural sources. In this sense, plants of the Amaryllidaceae family are characterized for a wide range of biological activities, including; antiviral, antibacterial, antiparasitic, psychopharmacological, among others. The aim of this work was to evaluate the antibacterial activity of alkaloids extract obtained from Rhodophiala araucana bulbs against Gram-negative and Gram-positive bacteria and identify the alkaloids presents. The bulbs of R. araucana were obtained from the Tolhuaca National Park (IX region, Chile) in December 2016. The dried bulbs (138 g) were crushed and macerated with MeOH (3 x 2 L), the macerate was filtered and evaporated to dryness. The crude extract was acidified to pH 2 with H2SO4, neutral materials were removed using Et2O and CHCl3. The aqueous phase was basified up to pH 9 with NH4OH (25% v/v) and extracted with n-hexane, CHCl3, AcOEt:MeOH (3:1) and n- BuOH. The antibacterial activity was performed by disc diffusion method and the alkaloids composition by gas chromatography-mass spectrometry. Chromatographic analysis of the extracts showed the presence of the isoquinolinic alkaloids, highlighting the presence of alkaloids of the type Tazettine, Galanthamine and Lycorin. The results showed antibacterial activity of the butanol extracts against Gram-positive bacteria.es_CL
dc.language.isoenges_CL
dc.relationinstname: Conicyt
dc.relationreponame: Repositorio Digital RI2.0
dc.rightsinfo:eu-repo/semantics/openAccesses_CL
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
dc.titleAntibacterial activity of alkaloids extract from Rhodophiala araucana (Amaryllidaceae)es_CL
dc.typePonencia
dc.identifier.folio1141245es_CL
dc.country.isoChilees_CL
dc.relation.ncongresses_CL
dc.relation.projectidinfo:eu-repo/grantAgreement//1141245es_CL
dc.relation.setinfo:eu-repo/semantics/dataset/hdl.handle.net/10533/93486
dc.type.driverinfo:eu-repo/semantics/lecture
dc.relation.namecongInternational Workshop Advances In Science And Technology Of Bioresourceses_CL
dc.date.startcongress2017-11-29
dc.date.endcongress2017-12-01


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